Homopolymers consist of a stretch of repeated nucleotides (i.e. \(\geq 2\)) occurring at some point in the genome. For example, the sequence AAAA is a length 4 adenine homopolymer. In the complete human genome assembly (CHM13 v1.1 from the T2T consortium4), 50% of its three gigabases are in homopolymers of size 2 or more, and 10% are in homopolymers of length equal or greater than 4. As can be seen in Figure 1.4, short and medium length homopolymers make up a significant part of the genome. In a previous GRCh38 human genome assembly, more than 1.9 megabases are in homopolymers of length 8 or higher98, representing about 1‰ of that assembly. The longest homopolymer run in the CHM13 v1.1 assembly is 86 (90 in GRCh3898).
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Number (1): Everything in this space must add up to 1. The answer is 1-1, placed horizontally.
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